Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Parasite-mediated alteration of behaviour and biomolecular dynamics in a mouse model

doi: 10.3389/fcimb.2025.1574660

Figure Lengend Snippet: Effects of conditioned Blastocystis media on serotonin synthesis by RIN14B cells, measured in concentration of serotonin in media after 24 hours. (A) Shows effects without Tph1 inhibitor, while (B) shows effects with the inhibitor. Statistical tests performed were ordinary one-way ANOVAs, n=3 for all samples except RIN14B only control, where n=11. Control data in both (A, B) is the same. ns indicates no significance, while * = p ≤ 0.05 and **** = p ≤ 0.0001.

Article Snippet: These cell lines have not been seen in publication since the late 2000’s, and are not maintained by the ATCC ( ). shows the effects of Blastocystis -conditioned media on serotonin levels in RIN14B cell media.

Techniques: Concentration Assay, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Parasite-mediated alteration of behaviour and biomolecular dynamics in a mouse model

doi: 10.3389/fcimb.2025.1574660

Figure Lengend Snippet: Effects of conditioned Blastocystis media on serotonin-associated gene expression in RIN14B cells. Each subfigure shows the changes induced by Blastocystis media conditioned with a different treatment - ST7B supernatant (A) , indole (B) , tryptophan (C) , and indolized ST7B supernatant (D) and (E) . Each point represents the expression level of a gene relative to a control dataset, with the exception of subfigure (E) , which is normalized to subfigure (B) . The x-axis shows fold change in gene expression, while the y-axis shows statistical significance. The horizontal line above the x-axis on each chart shows the p=0.05 cutoff. Points above this line are statistically significant. The three vertical lines represent fold change cutoffs of -1.5, 0, and 1.5, from left to right. Subfigure (A) contains highlighted regions demonstrating which regions of each chart contain points that exceed the cutoffs, and are therefore significantly changed by the conditioned Blastocystis media.

Article Snippet: These cell lines have not been seen in publication since the late 2000’s, and are not maintained by the ATCC ( ). shows the effects of Blastocystis -conditioned media on serotonin levels in RIN14B cell media.

Techniques: Gene Expression, Expressing, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Parasite-mediated alteration of behaviour and biomolecular dynamics in a mouse model

doi: 10.3389/fcimb.2025.1574660

Figure Lengend Snippet: Effects of conditioned Blastocystis media on serotonin synthesis by RIN14B cells, measured in concentration of serotonin in media after 24 hours. (A) Shows effects without Tph1 inhibitor, while (B) shows effects with the inhibitor. Statistical tests performed were ordinary one-way ANOVAs, n=3 for all samples except RIN14B only control, where n=11. Control data in both (A, B) is the same. ns indicates no significance, while * = p ≤ 0.05 and **** = p ≤ 0.0001.

Article Snippet: RIN14B cells (Cat. No. CRL-2059, ATCC, RRID: CVCL_3583) were seeded on 6-well plates (Cat. No. 657160, Greiner, USA) overnight.

Techniques: Concentration Assay, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Parasite-mediated alteration of behaviour and biomolecular dynamics in a mouse model

doi: 10.3389/fcimb.2025.1574660

Figure Lengend Snippet: Effects of conditioned Blastocystis media on serotonin-associated gene expression in RIN14B cells. Each subfigure shows the changes induced by Blastocystis media conditioned with a different treatment - ST7B supernatant (A) , indole (B) , tryptophan (C) , and indolized ST7B supernatant (D) and (E) . Each point represents the expression level of a gene relative to a control dataset, with the exception of subfigure (E) , which is normalized to subfigure (B) . The x-axis shows fold change in gene expression, while the y-axis shows statistical significance. The horizontal line above the x-axis on each chart shows the p=0.05 cutoff. Points above this line are statistically significant. The three vertical lines represent fold change cutoffs of -1.5, 0, and 1.5, from left to right. Subfigure (A) contains highlighted regions demonstrating which regions of each chart contain points that exceed the cutoffs, and are therefore significantly changed by the conditioned Blastocystis media.

Article Snippet: RIN14B cells (Cat. No. CRL-2059, ATCC, RRID: CVCL_3583) were seeded on 6-well plates (Cat. No. 657160, Greiner, USA) overnight.

Techniques: Gene Expression, Expressing, Control

Journal: Applied and Environmental Microbiology

Article Title: Serotonergic and immunomodulatory properties of the psychobiotic candidate Bacteroides finegoldii UO.H1052 and its extracellular vesicles

doi: 10.1128/aem.00891-25

Figure Lengend Snippet: B. finegoldii UO.H1052-derived postbiotics modulate serotonin metabolic pathways in enteroendocrine cells. RT-qPCR analysis of Tph1 and Maoa gene expression in RIN14B enteroendocrine cells after 6-h treatment with CFS and EVs. Gene expression was calculated relative to the FAB medium control. PBS served as a vehicle/background control using the ΔΔCt method and was normalized to the β-actin housekeeping gene. Both CFS and EVs induced differential transcriptional regulation of serotonergic markers.

Article Snippet: To investigate whether B. finegoldii UO.H1052 CFS and EVs could induce serotonin production in RIN14B cells, confluent RIN14B cells (ATCC) were used as a model for serotonergic activity.

Techniques: Derivative Assay, Quantitative RT-PCR, Gene Expression, Control